Journal: Poultry Science

Article Title: Establishment of a stable replicon cell line of Tembusu virus (TMUV) for high-throughput antiviral screening

doi: 10.1016/j.psj.2025.106109

Figure Lengend Snippet: Generation of Vero cell line expressing TMUV replicon. (A) The schematic flowchart delineates the key steps required for the establishment of a stable replicon-harboring cell line. (B) Vero cells were electroporated with viral replicon genomic RNA (10 μg) and BSD was supplemented into the medium 2 days later. Gluc activity was quantified in cell culture supernatants at indicated time points. (C) At 30 days post-electroporation, the intracellular total RNA was extracted and RT-qPCR assay was conducted to quantify viral RNA levels. Data represents the mean ± SD ( n = 3). *** p < 0.001. Statistical significance was assessed by two-tailed t -test. LOD represents limit of detection.

Article Snippet: Single electrical pulse was given with the Gene Pulser Xcell Electroporation Systems (Bio-Rad) with setting of 270 V at 950 microfarads.

Techniques: Expressing, Activity Assay, Cell Culture, Electroporation, Quantitative RT-PCR, Two Tailed Test